Refine your search
Co-Authors
Journals
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Das, Pradip Kumar
- DNA Barcoding of Domestic Indigenous Fowls from Eastern India
Abstract Views :538 |
PDF Views:1
Authors
Affiliations
1 West Bengal University of Animal and Fishery Sciences, Kolkata-700037, West Bengal, IN
2 Department of Biotechnology, Assam University, Silchar-788011, Assam, IN
1 West Bengal University of Animal and Fishery Sciences, Kolkata-700037, West Bengal, IN
2 Department of Biotechnology, Assam University, Silchar-788011, Assam, IN
Source
Journal of Environment and Sociobiology, Vol 10, No 1 (2013), Pagination: 7-14Abstract
Poultry biodiversity widely prevails over the world, especially in Indian subcontinent as domestic indigenous fowl. Cross species hybridization of different Gallus species confers ambiguity in proper classification. Molecular approach using DNA barcoding to identify species boundaries is successfully employed to detect hybridization signal of nondescript indigenous fowls. In the present study DNA barcoding region of cytochrome oxidase I (COI) gene collected from geographically distant region of five agroclimatic zones of eastern India is amplified using PCR, sequenced and subsequently deposited in GenBank. Related sequences were retrieved from global databases for phylogenic analysis using bioinformatics. The present study provides for the first time the DNA barcodes of domestic fowls from eastern India and established that the domestic fowls are grouped within the group Gallus gallus. Additional COI sequences within the same and related genus collected from different countries properly discriminate and assign their correct taxonomic positions. Low level of interspecies variation was observed between Gallus gallus and Gallus sonneratii indicating the failure to discriminate them as separate species.Keywords
DNA Barcoding, Poultry, Gallus Gallus, MtDNA, Cytochrome Oxidase I, Genbank.- Grid Failure
Abstract Views :210 |
PDF Views:102
Authors
Affiliations
1 Government of West Bengal, IN
1 Government of West Bengal, IN
Source
Reason-A Technical Journal (Formerly Reason-A Technical Magazine), Vol 2 (2001), Pagination: 18-20Abstract
Grid is a link which connects all power plants alongwith their connected loads under the same network in a particular region. For e. g. the Eastern Regional Grid of India consists of all power plants in West Bengal, Assam, Bihar, Orissa, Sikkim, i. e. WBSEB, BSEB, OSEB, ASEB, CHUKHA, DVC, DEL, CESC, etc.- Association Between Udder Morphology and In Vitro Activity of Milk Leukocytes in High Yielding Crossbred Cows
Abstract Views :152 |
PDF Views:0
Materials and Methods: A total of 48 healthy high yielding crossbred cows were selected for the study. The udder configuration and teat/udder morphology were recorded before milking. Milk samples (100 ml/cow) were collected aseptically. Milk somatic cell counts (SCC) and milk differential leukocyte counts were performed microscopically. Milk leukocytes (viz., neutrophils, lymphocytes, and macrophages) were isolated from milk samples by density gradient centrifugation. Phagocytic index (PI) of milk neutrophils and macrophages were evaluated by colorimetric nitro blue tetrazolium assay. Lymphocytes proliferation response was estimated by MTT assay and expressed as stimulation index.
Results: There was a significant (p<0.01) positive correlation between milk SCC with mid teat diameter, teat base diameter and significant (p<0.05) negative correlation between milk SCC and the height of the teat from the ground. Milk SCC was found to be significantly (p<0.01) lower in bowl-shaped udder and higher (p<0.01) in pendulous type. Milk macrophage percentage was positively (p<0.01) correlated with udder circumference. PI of milk neutrophil was negatively (p<0.01) correlation between teat base diameter, and PI of milk macrophages was found to be positively (p<0.01) correlated with teat apex diameter. Both PI of milk neutrophils and macrophages was found to be significantly (p<0.01) lower in the animals having flat and round teat and pendulous type of udder. In vitro PI of milk neutrophils was found to be significantly (p<0.01) lower in flat teat. In vitro PI of milk macrophages was found to be significantly (p<0.01) lower in the round and flat teats compared to pointed and cylindrical teats.
Conclusion: Udder risk factors such as teat shape and size, teat to floor distance, udder shape, and size may decrease the in vitro activity of milk leukocytes hence facilitates the incidence intramammary infections.
Authors
Affiliations
1 Department of Veterinary Physiology, West Bengal University of Animal and Fishery Sciences, Kolkata-700037, West Bengal, IN
1 Department of Veterinary Physiology, West Bengal University of Animal and Fishery Sciences, Kolkata-700037, West Bengal, IN
Source
Veterinary World, Vol 10, No 3 (2017), Pagination: 342-347Abstract
Aim: The present investigation was conducted to study the association between udder morphology and in vitro activity of milk leukocytes in high yielding crossbred cows.Materials and Methods: A total of 48 healthy high yielding crossbred cows were selected for the study. The udder configuration and teat/udder morphology were recorded before milking. Milk samples (100 ml/cow) were collected aseptically. Milk somatic cell counts (SCC) and milk differential leukocyte counts were performed microscopically. Milk leukocytes (viz., neutrophils, lymphocytes, and macrophages) were isolated from milk samples by density gradient centrifugation. Phagocytic index (PI) of milk neutrophils and macrophages were evaluated by colorimetric nitro blue tetrazolium assay. Lymphocytes proliferation response was estimated by MTT assay and expressed as stimulation index.
Results: There was a significant (p<0.01) positive correlation between milk SCC with mid teat diameter, teat base diameter and significant (p<0.05) negative correlation between milk SCC and the height of the teat from the ground. Milk SCC was found to be significantly (p<0.01) lower in bowl-shaped udder and higher (p<0.01) in pendulous type. Milk macrophage percentage was positively (p<0.01) correlated with udder circumference. PI of milk neutrophil was negatively (p<0.01) correlation between teat base diameter, and PI of milk macrophages was found to be positively (p<0.01) correlated with teat apex diameter. Both PI of milk neutrophils and macrophages was found to be significantly (p<0.01) lower in the animals having flat and round teat and pendulous type of udder. In vitro PI of milk neutrophils was found to be significantly (p<0.01) lower in flat teat. In vitro PI of milk macrophages was found to be significantly (p<0.01) lower in the round and flat teats compared to pointed and cylindrical teats.
Conclusion: Udder risk factors such as teat shape and size, teat to floor distance, udder shape, and size may decrease the in vitro activity of milk leukocytes hence facilitates the incidence intramammary infections.